https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38936 Wed 30 Aug 2023 14:20:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43721 Wed 28 Sep 2022 10:29:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39561 Wed 27 Jul 2022 14:35:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40462 Wed 27 Jul 2022 11:59:24 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45204 Wed 26 Oct 2022 15:29:09 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52622 Wed 13 Mar 2024 14:07:06 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6607 Wed 11 Apr 2018 15:33:34 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15122 Wed 11 Apr 2018 15:13:29 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14944 Wed 11 Apr 2018 14:30:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25775 Wed 11 Apr 2018 13:20:33 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38140 Wed 04 Aug 2021 15:49:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49743 Tue 30 May 2023 15:29:49 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54524 Tue 27 Feb 2024 19:29:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42488 Chlamydia infection, in both females and males, is increasing worldwide. Male infections have been associated clinically with urethritis, epididymitis, and orchitis, believed to be caused by ascending infection, although the impact of infection on male fertility remains controversial. Using a mouse model of male chlamydial infection, we show that all the major testicular cell populations, germ cells, Sertoli cells, Leydig cells, and testicular macrophages can be productively infected. Furthermore, sperm isolated from vas deferens of infected mice also had increased levels of DNA damage as early as 4 weeks post-infection. Bilateral vasectomy, prior to infection, did not affect the chlamydial load recovered from testes at 2, 4, and 8 weeks post-infection, and Chlamydia-infected macrophages were detectable in blood and the testes as soon as 3 days post-infection. Partial depletion of macrophages with clodronate liposomes significantly reduced the testicular chlamydial burden, consistent with a hematogenous route of infection, with Chlamydia transported to the testes in infected macrophages. These data suggest that macrophages serve as Trojan horses, transporting Chlamydia from the penile urethra to the testes within 3 days of infection, bypassing the entire male reproductive tract. In the testes, infected macrophages likely transfer infection to Leydig, Sertoli, and germ cells, causing sperm DNA damage and impaired spermatogenesis.]]> Tue 23 Aug 2022 15:34:32 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43154 Tue 13 Sep 2022 15:35:30 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38608 Chlamydia is the most common sexually transmitted bacterial pathogen worldwide. Male and female infections occur at similar rates and both cause serious pathological sequelae. Despite this, the impact of chlamydial infection on male fertility has long been debated, and the effects of paternal chlamydial infection on offspring development are unknown. Using a male mouse chronic infection model, we show that chlamydial infection persists in the testes, adversely affecting the testicular environment. Infection increased leukocyte infiltration, disrupted the blood:testis barrier and reduced spermiogenic cell numbers and seminiferous tubule volume. Sperm from infected mice had decreased motility, increased abnormal morphology, decreased zona-binding capacity, and increased DNA damage. Serum anti-sperm antibodies were also increased. When both acutely and chronically infected male mice were bred with healthy female mice, 16.7% of pups displayed developmental abnormalities. Female offspring of chronically infected sires had smaller reproductive tracts than offspring of noninfected sires. The male pups of infected sires displayed delayed testicular development, with abnormalities in sperm vitality, motility, and sperm-oocyte binding evident at sexual maturity. These data suggest that chronic testicular Chlamydia infection can contribute to male infertility, which may have an intergenerational impact on sperm quality.]]> Thu 18 Nov 2021 11:59:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:8340 Sat 24 Mar 2018 10:47:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:9576 Sat 24 Mar 2018 08:34:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1079 Sat 24 Mar 2018 08:32:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15274 Sat 24 Mar 2018 08:24:12 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10153 Sat 24 Mar 2018 08:07:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19950 Sat 24 Mar 2018 07:58:32 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29 Sat 24 Mar 2018 07:42:18 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27535 Sat 24 Mar 2018 07:28:58 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48243 Sat 11 Mar 2023 12:51:09 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54975 Mon 25 Mar 2024 15:21:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46955 Mon 12 Dec 2022 09:05:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33479 Mon 05 Nov 2018 11:10:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36447 Mon 04 May 2020 16:14:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45427 Fri 28 Oct 2022 11:48:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42487 Chlamydia be found in the testes of infertile men? Summary Answer: Chlamydia can be found in 16.7% of fresh testicular biopsies and 45.3% of fixed testicular biopsies taken from a selection of infertile men. What is known already: Male chlamydial infection has been understudied despite male and female infections occurring at similar rates. This is particularly true of asymptomatic infections, which occur in 50% of cases. Chlamydial infection has also been associated with increased sperm DNA damage and reduced male fertility. Study Design, Size, Duration: We collected diagnostic (fixed, n = 100) and therapeutic (fresh, n = 18) human testicular biopsies during sperm recovery procedures from moderately to severely infertile men in a cross-sectional approach to sampling. Participants/Materials, Setting, Methods: The diagnostic and therapeutic biopsies were tested for Chlamydia-specific DNA and protein, using real-time PCR and immunohistochemical approaches, respectively. Serum samples matched to the fresh biopsies were also assayed for the presence of Chlamydia-specific antibodies using immunoblotting techniques. Main Results and the Role of Chance: Chlamydial major outer membrane protein was detected in fixed biopsies at a rate of 45.3%. This was confirmed by detection of chlamydial DNA and TC0500 protein (replication marker). C. trachomatis DNA was detected in fresh biopsies at a rate of 16.7%, and the sera from each of these three positive patients contained C. trachomatis-specific antibodies. Overall, C. trachomatis-specific antibodies were detected in 72.2% of the serum samples from the patients providing fresh biopsies, although none of the patients were symptomatic nor had they reported a previous sexually transmitted infection diagnosis including Chlamydia. Limitations, Reasons for Caution: No reproductively healthy male testicular biopsies were tested for the presence of Chlamydia DNA or proteins or Chlamydia-specific antibodies due to the unavailability of these samples. Wider Implications for the Findings: Application of Chlamydia-specific PCR and immunohistochemistry in this human male infertility context of testicular biopsies reveals evidence of a high prevalence of previously unrecognised infection, which may potentially have a pathogenic role in spermatogenic failure.]]> Fri 26 Aug 2022 09:47:49 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51228 Fri 25 Aug 2023 11:51:38 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:55389 Fri 24 May 2024 10:34:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39668 n = 124) and a group with seminal abnormalities (SAG, n = 136). Evaluation included complete physical examination, past medical history, habits and lifestyle factors, two complete seminal analysis with sperm functional tests, serum levels of 25-hydroxy-vitamin D₃(25(OH)VD₃), total and free testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), sex hormone-binding globulin (SHBG), total cholesterol, homeostatic model assessment of insulin resistance (HOMA-IR) index, and karyotype. The mean concentration of 25(OH)VD₃ was significantly lower in the SAG (P < 0.001) and positively correlated with all baseline seminal parameters and total testosterone levels. In addition, serum vitamin D₃ concentration was found to be positively correlated with sperm concentration (β = 2.103; P < 0.001), total number of spermatozoa with progressive motility (β = 2.069; P = 0.003), total number of motile spermatozoa (β = 2.571; P = 0.015), and strict morphology (β = 0.056; P = 0.006), regardless of other variables. This is the first comparative study to address the issue of serum vitamin D₃ content between normozoospermic patients and those with sperm abnormalities. It clearly demonstrates a direct and positive relationship between serum vitamin D level and overall semen quality, male reproductive potential, and testosterone levels.]]> Fri 17 Jun 2022 15:25:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45727 Fri 04 Nov 2022 09:52:06 AEDT ]]>